starrylizard: Headshot of Ruby from Demons smiling (T- Do go on...  (O'Neill))
starrylizard ([personal profile] starrylizard) wrote2006-12-15 11:34 am
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Writing the methods section makes [personal profile] starrylizard a very dull girl

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lark_ascends: Blue and purple dragonfly, green background (Default)

[personal profile] lark_ascends 2006-12-15 01:11 am (UTC)(link)
*snorts*

[identity profile] starrylizard.livejournal.com 2006-12-15 01:29 am (UTC)(link)
The culture medium was changed to serum-free medium 24 hours before the phosphate uptake assay was to be carried out. For transfection experiments, cells were subcultured into 24-well plates at a density of approximately 60 000 to 100 000 cells per well in a volume of 500 µL per well. The culture medium was changed every 2 to 4 days until the cells had reached ~80% confluence and the transfection was carried out in Optimem (Gibco BRL Life Technologies, Gaithersburg, MA, USA) supplemented with 2% FCS (v/v) and...

Seriously! SO bored!
I remember that movie scene so badly. Good old Jack Nicholson! I think I was far to young to be watching that movie. :P